Molecular epidemiological and clinical features of human bocavirus in children with acute respiratory tract infection in Shanghai
10.3760/cma.j.issn.1003-9279.2016.05.009
- VernacularTitle:上海地区人博卡病毒在儿童急性呼吸道感染的研究
- Author:
Jingrong SONG
1
;
Hanchun GAO
;
Zhen LIN
;
Juan KANG
;
Jianmin YE
;
Zizhen YANG
;
Zhiping XIE
Author Information
1. 201900,上海交通大学医学院附属第九人民医院儿科
- Keywords:
Respiratory tract infection;
Human bocavirus;
Epidemiology;
Child
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(5):452-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiology and clinical characteristics of human bocavirus in children with acute respiratory tract infection in Shanghai,China.Methods Between January 2012 and December 2012,271 nasopharyngeal aspiration (NPA) samples were collected from children who had been hospitalized for acute respiratory tract infection at Shanghai,China.HBoV NS1 gene was detected by the nested polymerase chain reaction.In the further,other common respiratory viruses (HRV,RSV,ADV et al) was screened in HBoV positive samples,All PCR positive products were sequenced and phylogenetic analysis.Results The overall frequency of HBoV,infection was 11.4％.21of 31 HBoV positive sample tested were mix-infection.HBoV was detected from all over year.Children with HBoV varied in age from 4 months to 4-years (median age,17 months).The clinical diagnoses of HBoV positive patients included acute upper respiratory tract infection and lower respiratory tract infection.The clinical presentations of HBoV positive children included fever,cough,sputum production,diarrhea,vomiting;pharynx engorgement,crackles,wheezing.There being a statistically significant difference in the detection rates of HBoV between the outpatients and inpatients.The HBoV NS1 gene sequences phylogenetic analysis revealed that 29 HBoV1 NS1 sequences shared 99％-100％ nucleotide identity with the human bocavirus strain,whereas the amino acid identity was 96％-100％.The two HBoV2 NS1 sequences shared 99％ nucleotide sequence identity with HBoV2 strain FJ170282 respectively.Conclusions Human bocavirus was distributed in Shanghai region,HBoV1 was dominant and HBoV2 was detected at the first time.The symptom and clinical diagnoses has no specificity with patients with other common respiratory viruses.
