Genetic characteristics of rubella virus isolated in Sichuan province in 2015
10.3760/cma.j.issn.1003-9279.2016.05.003
- VernacularTitle:2015年四川省风疹病毒分离株的基因特征分析
- Author:
Li LIU
1
;
Jilan HE
;
Xiaozhen MA
;
Ranran CAO
;
Yulan HUANG
Author Information
1. 四川省疾病预防控制中心
- Keywords:
Rubella virus;
Sequence analysis;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(5):425-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the genetic characteristics of rubella virus in Sichuan province in 2015.Methods Veto/SLAM cells were used for rubella virus isolation and culture.The 739 nucleotides of E1 gene was amplified by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and subjected to sequence and homological analysis.Results 19 strains of rubella virus were isolated and identified as genotype 2B.The homology of nucleotides and amino acids were 99％-100％ and 97.9％-100％,respectively.There were no changes in the important antigenic epitopes.Conclusions The rubella virus genotype circulated in Sichuan province in 2015 was genotype 2B.
