Molecular epidemiological characteristics of measles virus in Sichuan province in 2015

VernacularTitle:2015年四川省麻疹病毒分子流行病学特征分析
Author: Ranran CAO ¹ ; Li LIU ; Yukun ZHU ; Shundong WANG ; Jilan HE
Author Information

1. 四川省疾病预防控制中心
Keywords: Measles virus; Nucleoproteins; Genotype
From: Chinese Journal of Experimental and Clinical Virology 2016;30(4):378-381
CountryChina
Language:Chinese
Abstract: Objective To analyze the genetic characteristics of measles virus in Sichuan province in 2015.Methods Measles virus was isolated from throat swab specimens collected from suspected measles cases.Nucleotide sequences coding for C terminus of nucleoprotein were amplified by RT-PCR and sequenced for phylogenetic analysis.Results 398 measles virus isolates were obtained from 971 throat swab specimens.Phylogenetic analysis showed that 397 belonged to H1 genotype,the sequences homologies were 96.2％-98.0％ compared with H1 genotype reference strain;and all those isolates separated into 2 clusters in phylogenetic tree with the average nucleotide divergence of 2.0％.1 isolate belonged to A genotype,and shared 98.0％ nucleotide homology compared with A genotype reference strain.Conclusions H1 genotype virus remained predominant circulating in Sichuan Province.There was no much genetic variation in N gene.But a minor nucleotide divergence existed between different clusters,leading to 2 transmission chains.