Effects of hepatitis E virus open reading frame 3 protein on the proliferation and cell cycle of Chang liver cells
10.3760/cma.j.issn.1003-9279.2016.04.009
- VernacularTitle:戊型肝炎病毒ORF3蛋白对人肝细胞CCL13增殖及细胞周期的影响
- Author:
Lin CHEN
1
;
Yuhe LIANG
;
Xiaojun YANG
;
Xinhui YUAN
;
Zhiwu LIU
;
Hong YUAN
;
Peng GAO
Author Information
1. 730000,兰州大学第一医院传染科
- Keywords:
Heptitis E;
ORF3;
Proliferation;
Cell cycle
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(4):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hepatitis E virus Open Reading Frame 3 protein on the proliferation and cell cycle of Chang liver cells (CCL13).Methods The pDsRed-Monomer-N1-ORF3 recombinant plasmid was transfected into CCL13 cells via lipofectamine 2 000 reagent,then assayed cell proliferation by MTT assay;Flow cytometric was used to detect cell cycle after dying with PI;and the cell cycle proteins expression were detected by western-blotting.Results pDsRed-Monomer-N1-ORF3 recombinant plasmid was successfully transfected into CCL13 cells,Expression of ORF3 can inhibit the proliferation activity of CCL13 cells from the 3rd day (P ＜ 0.01),and prevent the cell cycle in G0/G1 phase;Western Blot showed expression of cell cycle proteins cyclin D1 and cyclin E1 were decreased in ORF3 group compared with the control group;However,the expression of cyclin A2 and cyclin B1 had no significantly difference in three groups.Conclusions HEV-ORF3 can inhibits the proliferation activity of CCL13 cells by inhibition expression of cell cycle proteins cyclin D1 and cyclin E1.
