Molecular characterization analysis of one H5N6 subtype influenza virus
10.3760/cma.j.issn.1003-9279.2016.04.006
- VernacularTitle:1株H5N6亚型禽流感病毒分子特征分析
- Author:
Huiyan YU
1
;
Fei DENG
;
Shenjiao WANG
;
Xiang HUO
;
Qigang DAI
;
Xian QI
Author Information
1. 江苏省疾病预防控制中心急性传染病防治所
- Keywords:
Avian influenza virus;
H5N6 subtype;
gene reassortment
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(4):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective One H5N6 subtype avian influenza virus,A/environment/ Zhenjiang/C13/ 2013,was isolated from environment in Zhenjiang in 2013 during epidemiological surveillance and was identified by HA/HI test and specific RT-PCR test.H5N6 AIV was subjected to genome sequencing to investigate the genomic composition.Methods The next-generation sequencing (NGS) Illumina MiSeq Platform was used to obtain complete genome sequence information from influenza virus isolates.MEGA6.0 software was used to align the eight fragments of the virus.The phylogenetic and molecular characteristics of the isolate were analyzed by Neighbor-Joining method.Results Blast searches demonstrated that the NA gene belonged to the same clade with H6N6 viruses currently circulating in China,whereas the other seven genes were found to be more similar to those of eastern Asian H5N1 AIV strains gene.The amino acid motif of cleavage site between HA1 and HA2 was PLREKRRKR ↓ GLF,which was the typical characteristics of the HPAIVs.The receptor binding site in the viral HA gene possesses the residues Gln 226 and Gly 228 (H3 numbering),indicating an avian-like receptor binding specificity.Conclusion One H5N6 subtype avian influenza virus was isolated from environment,phylogenetic analysis showed that the NA gene belonged to the same clade with H6N6 viruses.The other seven genes belonged to H5N1 subtype.It suggests that strengthen the continuous surveillance of avian influenza A viruses is necessary.
