Molecular epidemiological analysis of dengue virus in Haizhu district of Guangzhou from 2012 to 2015
10.3760/cma.j.issn.1003-9279.2016.04.003
- VernacularTitle:广州市海珠区2012-2015年登革病毒分子流行病学分析
- Author:
Pengjuan GUO
1
;
De WU
;
Huan ZHANG
;
Huiqiong ZHOU
;
Jinhua TAN
;
Shaohong XU
Author Information
1. 510288,广州市海珠区疾病预防控制中心
- Keywords:
Dengue virus;
Gene;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(4):346-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular characteristic and trace the resource of dengue virus in Zhuhaidistrict of Guangzhou during 2012-2015.Methods Collected the cases data of dengue fever in Zhuhai district from 2012-2015 and analyzed the epidemical characteristic.DENV strains were isolated by C6/36 cells,the E gene was amplified from the positive specimen by RT-PCR.The PCR products were sequenced and then analyzed by bio-information software.Results Total of 6 260 DENV infection cases were reported,and the cases happened in every age group;57.78％ of the cases occurred in October.16 virus strains were isolated from 48 samples and the whole E genes were successfully amplified,the virus strains from 2013 and 2014 were the same one and the nucleotide sequence 99.93％ identify with DENV-1 in 2009.Phylogenetic analysis showed that DENV-1 belonged to the G1 genotype,genetically close to the strains from Thailand;strains from 2015 were the same one and belonged to the Cosmopolitan genotype of DENV-2,most similar with the strains from India.Conclusions The DENV-1 outbreak in Haizhu district of Guangzhou during 2012-2015 were belonged to G1 that originated from Thailand and might indigenous in 2009;DENV-2 was belonged to Cosmopolitan genotype which was imported.
