Analysis of endogenous viral elements derived from dengue virus in Aedes albopictus genome
10.3760/cma.j.issn.1003-9279.2016.04.001
- VernacularTitle:白纹伊蚊内源性登革病毒序列分析
- Author:
Yanhai WANG
1
;
Jinbao GU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
- Keywords:
Dengue virus;
Aedes;
Endogenous viral elements
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(4):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and analyze dengue virus-derived endogenous viral elements (EVEs) in Aedes albopictus (Foshan strain).Methods The full sequences of four serotypes of dengue virus (DENV 1-4) were used as queries,and Blast N program was used to scan the genome of Ae.albopictus.The candidates of EVEs were confirmed by PCR method using specific primers with genomic DNA as template from 8 individuals of female and male adult,respectivly.Results Total 5 EVEs candidates displayed the high genetic similarity with DENV genome sequences.Among them,EVE-1 showed 100％ genetic similarity with DENV-2 P8-1407MS strain,whereas EVE-2-5 showed more than 99％ genetic similarity with DENV-1 02-20 strain.Genomic DNA PCR results indicated that EVE-1 exhibit sequence conservation across individuals among population,whereas the EVE-3-5 displayed the genomic sequence variation in individuals.Conclusions Dengue virus-derived EVEs were indentified in Ae.albopictus,that may provide new insight into the interaction between DENV and their vectors.
