Impact of interferon induced transmembrane proteins IFITM1/2/3 on SFTS virus infection
10.3760/cma.j.issn.1003-9279.2016.03.006
- VernacularTitle:干扰素诱导的跨膜蛋白IFITM1/2/3对SFTS病毒感染的影响
- Author:
Jia WAN
1
;
Shuo ZHANG
;
Aqian LI
;
Jing QU
;
Chuan LI
;
Dexin LI
;
Mifang LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
IFITM1/2/3;
SFTS virus;
antiviral effect
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(3):279-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of IFITM1/2/3 on SFTS virus infection.Methods IFITM1/2/3 expression vectors were constructed by inserting corresponding genes into pCAGEN vector respectively and the expression of IFITM1/2/3 were detected by immunofluorescence assay (IFA) and Western Blot analysis.SFTS virus load at different time post infection on Vero and THP-1 cells that overexpressing IFITM1/2/3 were determined by quantitative real-time RT-PCR.Results High intracellular antibody binding activities of IFITM 1/2/3 were observed by IFA and Western Blot assay.The results of realtime RT-PCR indicated that IFITM1/2/3 could significantly inhibit the infection of SFTS virus on the above two cell lines.Furthermore,at early stage of virus infection,the inhibition effects were more obvious for low dose infection.Conclusions IFITM1/2/3 could effectively inhibit intracellular SFTS virus infection.
