Intervention mechanism of Yangyin Yiqi Heji to alveolus epithel mesenchymal transition in rats with pulmonary fibrosis
10.3969/j.issn.1006-2157.2019.01.009
- VernacularTitle:养阴益气合剂对肺纤维化大鼠肺泡上皮间质转化的干预机制研究
- Author:
Lihong MENG
1
;
Hong WANG
;
Huan DONG
;
Xiaofeng GU
;
Xiaolin YU
;
Yushan LIU
;
Dayong XIN
;
Xinping QIU
;
Xiaomei ZHANG
Author Information
1. 北京中医药大学
- Keywords:
Yangyin Yiqi Heji (Yin-nourishing Qi-replenishing Mixture);
pulmonary fibrosis;
epithelial-mesenchymal transition;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2019;42(1):52-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the interventional effect of Yangyin Yiqi Heji (Yin-nourishing Qireplenishing Mixture) to alveolus epithelial-mesenchymal transition in rats with bleomycin-induced pulmonary fibrosis.Methods Wistar rats in SPF class (n=120) were randomly divided into normal group, model group, prednisone group and low-dose, mid-dose and high-dose Yangyin Yiqi Heji groups.The rat model of pulmonary fibrosis was copied by using intratracheal instillation of bleomycin, and all groups were orally given corresponding medicinals for 28 d after modeling for 1 d.The rats were executed respectively on the 14th d and 28th d to collect lung samples for detection of left lung pathological changes by using HE and Masson staining.The mRNA expressions ofα-smooth muscle actin (α-SMA) , E-cadherin, collagen I (Col I) and laminin (LN) were detected in rigth lung by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR).Results The mRNA expressions ofα-SMA, Col I and LN increased, and E-cadherin expressions decreased in model group compared with normal group (P<0.05).After treatment, the mRNA expressions ofα-SMA, Col I and LN decreased, and mRNA expression of E-cadherin increased in all treatment groups (P<0.05).On the 28 th d, mRNA expression of E-cadherin increased in high-dose Yangyin Yiqi Heji group compared with prednisone groups (P<0.05) , and mRNA expression of LN deecresed in high-dose, mid-dose and low-dose Yangyin Yiqi Heji groups (P<0.05).Conclusion Yangyin Yiqi Heji can inhibit the epithelial-mesenchymal transition in rats with bleomycin-induced pulmonary fibrosis, and the mechanism may be related to down-regulation of genes ofα-SMA, Col I and LN and up-regulation of E-cadherin gene.
