Effects of different antigen stimulation methods on the proliferation of HIV specific CD8 T cells
10.3760/cma.j.issn.1003-9279.2016.03.001
- VernacularTitle:不同抗原肽刺激方式对HIV特异性CD8T细胞体外增殖的影响
- Author:
Cong JIN
1
;
Ting TU
;
Danlei MOU
;
Qunhui LI
;
Tao LI
;
Hao WU
;
Huabiao CHEN
Author Information
1. 中国疾病预防控制中心性病艾滋病预防控制中心
- Keywords:
Acquired immunodeficiency syndrome;
CD8 T cells;
Ex vivo proliferation
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(3):257-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the factors that influence the ex vivo proliferation of human immunodeficiency virus (HIV) specific CD8 T cells.Methods Three methods to use HIV-specific antigen peptides stimulating the proliferation of virus specific CD8 T cells from HIV patients were compared,and the effect of adding exogenous IL-2 into co-culture on proliferation was evaluated.Results In comparison to directly adding MHC I matched HIV peptides into peripheral blood mononuclear cells (PBMCs) by presence during co-culture or incubating for 2 hours,the more efficient proliferation of HIV-specific CD8 T cells was stimulated by loading HIV peptides on the mixed antigen presenting cells (APCs) that harvested by removing CD8 T cells from PBMCs.Adding IL-2 into co-culture could largely enhance the number of proliferated HIV-specific CD8 T cells but also induced non-specific proliferation of CD8 T cells.Conclusions Stimulation of HIV-specific CD8 T cells by loading HIV-specific antigen peptides on mixed APCs and in absence of IL-2 can specifically induce the efficient proliferation of HIV-specific CD8 T cells by providing both antigen and co-stimulation signals.
