Impact of extracts of Xuanshenon liver metabolome in rat modelof hyperthyroidism
10.3969/j.issn.1006-2157.2019.01.005
- VernacularTitle:玄参提取物对甲亢模型大鼠肝脏代谢组学的影响
- Author:
Ning ZHANG
1
;
Zihui LI
;
Hongwei ZHAO
;
Mu PANG
;
Tao YE
;
Shumin LIU
Author Information
1. 黑龙江中医药大学药物安全性评价中心
- Keywords:
Xuanshen (Scrophularia Root,Radix Scrophulariae);
Euthyrox;
hyperthyroidism;
liver metabonomics;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2019;42(1):21-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and possible mechanism of extracts of Xuanshen (Scrophularia Root, Radix Scrophulariae) on liver metabolome in rat model of hyperthyroidism.Methods The rat liver metabolites spectrums effected by Euthyrox and Xuanshen extracts were analyzed by using ultra high performance liquid chromatography-time of-flight-mass spectrometry (UPLC-TOF-MS).The difference in metabolites spectrums was studied between blank group and model group by using orthogonal partial least squares-discriminant analysis (OPLS-DA).The potential biomarkers were found in liver through variable importance projection (VIP).The interventional effects of Xuanshen extracts were discussed through comparing the difference in content of potential biomarkers.Results There were 21 potential biomarkers found in rat liver in model group, and among them14 metabolites were significantly called back by Xuanshen extracts.The biosynthesis of glycosylphosphatidylinositol (GPI) -anchor, and metabolisms of arachidonic acid, purine, α-linolenic acid, linoleic acid, glutathione and glycerophospholipid were significantly improved by Xuanshen extracts in Euthyrox-induced hyperthyroidism rat model.Conclusion Xuanshen extracts can play a role of treating hyperthyroidism through improving relevant liver potential biomarkers and metabolism pathways in hyperthyroidism rat model.
