Primary establishment of a homogeneous enzyme immunosorbent assay for EV71 IgM of HFMD serum

VernacularTitle:EV71 IgM抗体均相酶联免疫检测方法的初步建立
Author: Yu CUI ¹ ; Ying WANG ; Xing GAN ; Juan SONG ; Jun HAN
Author Information

1. 450000,郑州人民医院
Keywords: EV71; VP1; IgM; homogeneous ELISA
From: Chinese Journal of Experimental and Clinical Virology 2016;30(2):220-222
CountryChina
Language:Chinese
Abstract: Objective To develop new homogeneous enzyme immunosorbent assay for serum IgM antibody of EV71 infection.Methods After protein EV71 VP1 1-297 was purified and biotinylated,the optimal quantities of the antigen was obtained by ELISA.The homogeneous enzyme immunosorbent assay was valuated with the serum samples of both 20 healthy subjects and 30 samples from the patients of HFMD detected by ELISA.Results The reaction system of homogeneous enzyme immunosorbent assay for EV71 IgM was established in this study.Then the homogeneous enzyme immunosorbent assay was valuated with ELISA for EV71 IgM.The results revealed serum IgM were negative for the 20 healthy subjects by both ELISA and homogeneous enzyme immunosorbent assay.Of the 30 HFMD patients,positive serum EV71 IgM detected by both ELISA and homogeneous enzyme immunosorbent assay,and yet,6 IgM positive samples were identified with homogeneous enzyme immunosorbent assay in the 14 negative samples detected by ELISA.Conclusion A homogeneous enzyme immunosorbent assay for detection of serum EV71 IgM has been established.