The mechanism of applying lysogenicity in phage-biotyping scheme for subtyping O1 E1 Tor Vibrio cholerae strains
10.3760/cma.j.issn.1003-9279.2016.02.022
- VernacularTitle:O1群E1Tor型霍乱弧菌溶原性测定的机理研究
- Author:
Xiaona SHEN
1
;
Jingyun ZHANG
;
Xiuping FU
;
Jie LI
;
Weili LIANG
;
Biao KAN
Author Information
1. 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
- Keywords:
Vibrio cholerae;
Phage-Biotyping Scheme;
lysogenic bacteriophage
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(2):199-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the principle of applying lysogenicity in Phage-Biotyping Scheme developed for the subtyping of O1 E1 Tor Vibrio cholerae strains.Methods 118 V.cholerae strains including 76 E1 Tor strains,8 classical strains and 34 serogroup O139 were selected to analyze the lysogenicity and sensitivity to lysogenic phage 919TP as described in the Manuals of cholera prevention and control,the genes of this phage were also determined among the genome sequences of these strains and the phages produced by them.Results All O1 E1Tor Vibrio cholerae 19 strains that produced positive results in lysogenicity,had the lysogenic K139 phage in genome and could both resist to lysogenic phage 919TP and release the K139 family phage.All the O1 E1Tor Vibrio cholerae 22 strains that produced positive results in sensitivity to the lysogenic phage,had no K139 family phage genes and got negative results in lysogenicity.However,the phages of this family were not released from 6 classical strains with positive lysogenicity result.Five serogroup O139 strains were detected releasing temperate phages K139 without the sensitivity to phage 919TP.Conclusions Applying the lysogenicity in Phage-Biotyping Scheme for subtyping O1 E1 Tor Vibrio cholerae strains is based on the ability to produce lysogenic bacteriophage K139.The index of "sensitivity to the lysogenic phage" was also associated with this ability.
