The T cell activation and cell factor's changes in enterovirus encephalitis children
10.3760/cma.j.issn.1003-9279.2016.02.015
- VernacularTitle:肠道病毒性脑炎的患儿T细胞活化亚群和细胞因子的变化
- Author:
Chunyan FANG
1
,
2
;
Xiaojie ZOU
;
Zhefeng YUAN
;
Minghai HUANG
;
Feng GAO
Author Information
1. 310000 杭州,浙江大学医学院附属儿童医院
2. 315000 宁波市妇女儿童医院
- Keywords:
Enterovirus Encephalitis;
CSF enterovirus DNA positive;
T cell activation subgroup;
Cell factor
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(2):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the action of immune function in Enterovirus Encephalitis (EVE) children by detecting the T cell activation and cell factors.Methods 24 infant patients with EVE hospitalized in the wards of department of neurology of the Children' s Hospital Affiliated to Medical School of Zhejiang University from June 2014 to July 2015 were selected as the experimental group,14 of the 24 patients were detected for the T cell activation.While another 49 healthy infants accepting physical check in the same period were selected as the control group.Flow cytometry was adopted to detect the peripheral blood CD25 +/CD3 + CD4 +,CD69 +/CD3 + CD4 +,HLA-DR +/CD3 + CD4 +,CD25 +/CD3 + CD8 +,CD69 +/CD3 + CD8 +,HLA-DR +/CD3 + CD8 +,and IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ levels of the two groups.Results The peripheral blood CD69/CD3 + CD4 +、CD69/CD3 + CD8 + expression levels (1.193％ ± 2.849％,0.696％ ± 1.242％) of the experiment group were lower than those of the control group (Z =-2.722,P=0.006;Z=-3.321,P=0.001).The expression level of the peripheral blood IL-2 (3.342 ± 1.089 pg/ml) was lower than that of the control group(Z =-2.722,P =0.019);while the expression of IL-6 and TNF-α (123.046 ± 355.349;3.367 ± 1.717pg/ml) were significantly higher than the control group (Z =-3.471,P =0.001;Z =-4.803,P ＜ 0.001) . Conclusion There is certain degree of immune dysfunction in EVE patients,T cell activation subgroup and the cell factors may participate in the morbidity progress of EVE.
