Molecular epidemiology of Norovirus from acute gastroenteritis outbreaks in Guangzhou
10.3760/cma.j.issn.1003-9279.2016.02.014
- VernacularTitle:2012-2013年广州市5起胃肠炎暴发中诺如病毒的分子流行病学特征
- Author:
Huaping XIE
1
;
Jinmei GENG
;
Jingwen LIU
;
Chun CHEN
;
Wenfeng CAI
;
Biao DI
;
Jun YUAN
Author Information
1. 510440,广州市疾病预防控制中心
- Keywords:
Norovirus;
Acute gastroenteritis;
Outbreak;
Sydney 2012 variant
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(2):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the molecular epidemiology of norovirus from 5 outbreaks of gastroenteritis during Dec.2012 to Jan.2013 in Guangzhou.Methods Epidemiologic data and specimens were collected from 5 gastroenteritis outbreaks in Guangzhou.274 specimens were detected for norovirus by RT-PCR methods and PCR products were sequenced.Sequence alignment and phylogenetic analysis were performed by using Clustal X 1.83 and MEGA 5.05 programs.Statistical analysis was performed by using SPSS 17.0 program.Results The total positive rate of norovirus was 20.07％ (55/274).The positive rate was 8.70％ (2/23) in University A,36.36％ (8/22) in Kindergarten B,36.07％ (22/61) in University C,100％ (5/5) in community health service center D,11.04％ (18/163) in University E.The positive rate was 100％ (6/6) among age group ≥ 60 years old,47.37％ (9/19) among 10-19 age group and 36.36％ (8/22) among age group ＜ 10 years old.Phylogenetic analysis showed that 33 samples were infected by the new variant-Sydney 2012 of norovirus GⅡ.4.Conclusion Norovirus was one of the main pathogens causing acute gastroenteritis outbreaks and GⅡ.4 Sydney 2012 variant was identified as the predominant strain in Guangzhou.
