Genetic characteristics of Coxsackie virus B5 isolates in Sichuan province over 2007-2014

VernacularTitle:2007-2014年柯萨奇病毒B5四川分离株的基因特征分析
Author: Xiaozhen MA ¹ ; Wenbin TONG ; Li LIU ; Ranran CAO ; Na CHEN
Author Information

1. 四川省疾病预防控制中心
Keywords: Acute flaccid paralysis; Coxsackie virus B5; Genetic characteristics analysis
From: Chinese Journal of Experimental and Clinical Virology 2016;30(2):157-160
CountryChina
Language:Chinese
Abstract: Objective To investigate genetic characteristics of Coxsackie virus B5 (CVBS) in acute flaccid paralysis(AFP) cases in Sichuan Province.Methods 10 CVB5 strains isolated from stool samples of AFP cases in Sichuan Province over 2007-2014 were subjected to entire VP1 coding region amplification by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing,and phylogenetic tree was constructed for genetic characterization.Results All of the 10 strains were identified as genogroup D.The nucleotide and the amino acid homologies were 80.4％-81.9％ and 95％-97.1％,which compared with the Faulkner prototype strain.The amino acid homologies between Ziyang strain and Chengdu strain obtained in 2014 were 100％,respectively.The nucleotide and the amino acid homologies between Nanchong isolate and Yibin isolate obtained in 2014 were both 100％,respectively.Conclusions The isolates from AFP cases in Sichuan Province over 2007-2014 were belong to genogroup D.The genetic characteristics of 10 strains were stable.