Complete genome sequence analysis of the Hantaan viruses isolated from Shaanxi Province and its Characteristics
10.3760/cma.j.issn.1003-9279.2016.02.008
- VernacularTitle:陕西省汉滩病毒分离株全基因组序列分析及生物学特性研究
- Author:
Yingxin MA
1
;
Pengbo YU
;
Quanfu ZHANG
;
Jiandong LI
;
Shiwen WANG
;
Dexin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hantavirus;
virus isolation;
peripheral blood mononuclear cell;
genome sequencing;
phylogenetic analysis;
microneutralization test
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(2):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To sequence the whole genome and to analyze the molecular and evolutionary of Hantavirus(HV) isolated from peripheral blood leukocytes (PBMC) of HFRS patients.Methods PBMC were isolated from peripheral blood of HFRS patients and cocultured with Vero-E6 cells.Quantitative realtime RT-PCR and IFA tests were used to detect virus titer.The genome segments were amplified by PCR with predesigned primers,whole sequence were obtained and further study on its evolution was analyzed.The differences between the isolate strains and Vaccine strain were tested by microneutralization test.Results Two Hantaan virus strains were isolated from PBMC of HFRS patients successfully.The whole genomic fragments were obtained.Molecular phylogenetic analysis result showed that both of the strains had the comparatively high similarity with XAAa10091712 strain.The seroneutralization titer of SX26 and Z10 had significant differences.Conclusion Hantaan virus could be successfully isolated from PBMC of HFRS patients,and it proved to be a feasible way of isolation of hantavirus from peripheral white blood cells;The microneutralization test proved that the antigenicity of two strains exist significant differences.It provided basis for the further study of hantavirus epidemic strains in this region.
