Effects of Zhige Jiejiu Baogan Fang on SOD,MDA and GSH in rats with alcoholic liver injury
10.3969/j.issn.1006-2157.2018.04.008
- VernacularTitle:枳葛解酒保肝方对酒精性肝损伤大鼠SOD、MDA、GSH的影响
- Author:
Feng CHEN
1
;
Dianna LIU
;
Shaohong CHEN
;
Haiyan LIU
;
Linlin XIU
;
Jie GAO
;
Na LI
;
Chen ZHANG
;
Gansheng ZHONG
Author Information
1. 北京中医药大学中医学院 北京100029
- Keywords:
Zhige hangover-relieving and liver-protecting recipe;
alcoholic liver injury;
superoxide dis-mutase;
malondialdehyde;
reduced glutathione;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(4):306-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Zhige Jiejiu Baogan Fang(Semen Hoveniae and Radix Puerariae Lobatae hangover-relieving and liver-protecting recipe, ZGJJBGF) on superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) rats with alcoholic liver injury. Methods 180 male Wistar rats were randomly divided into 6 groups: blank group, model group, positive control (Compound Methionine and Choline Bitartrate Tablet) group (0.36 g/kg), high-dose, mid-dose and low-dose ZGJJBGF groups (18,9,1.8 g/kg respectively). Except for the blank group, rats in other groups were orally administered with 50% ethanol(diluted with distilled water) for 6 weeks to establish the alcoholic liver injury rat model. Rats in all experimental groups except the blank and model groups received medicinal intervention respectively. The activity of malondialdehyde (MDA), glutathione (GSH),and superoxide dismutase(SOD) in serum and liver were measured at the end of the experiment. Results SOD activity in model group was significantly lower than that in the blank group, and the contents of MDA and GSH in the liver tissues and blood were significantly higher in the blank group (P<0.01). Compared with model group, mid-dose ZGJJBGF significantly increased the serum GSH level (P<0.05) and the activity of SOD (P<0.01) while reducing the content of MDA in liver tissue(P<0.01). Compared with the model group,high-dose ZGJJBGF significantly reduced the content of MDA in liver tissue of rats(P<0.01) and increased the content of GSH(P<0.01). Compared with model group, low-dose ZGJJBGF reduced serum MDA content (P<0.01). Conclusion Zhige Jiejiu Baogan Fang could enhance the antioxidant ability of endogenous antioxidant enzymes such as SOD and GSH. It can also inhibit the damage of liver injury of free radical-induced lipid peroxidation.
