Value of diagnosis on hepatitis C by ELISA-HCV core antigen detection
10.3760/cma.j.issn.1003-9279.2016.01.017
- VernacularTitle:核心抗原酶联免疫检测在丙型肝炎病毒感染诊断中的价值
- Author:
Weiyue CHEN
1
;
Yong YU
;
Peng DU
Author Information
1. 315000,宁波市中心血站
- Keywords:
Hepatitis C virus;
Core antigen;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2016;(1):64-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinically applied valve of HCV core antigen enzyme-linked immunosorbent assay ( HCV-cAg ELISA) for early diagnosis of hepatitis C.Methods Both HCV RNA and HCV-cAg ELISA were used to detect 83 cases of anti-HCV positive serum samples and 500 cases of anti-HCV negative but ALT abnormal serum samples.Results In the 83 cases of anti-HCV positive serum samples, 33 cases were detectable for HCV-cAg while 36 cases were detectable for HCV RNA, in which 32 cases were double positive for both HCV-cAg and HCV-RNA.In the 500 cases of anti-HCV negative but ALT abnormal serum sample, only 3 cases were detectable for both HCV-cAg and HCV RNA. The coincidence rates of the two methods for detecting the anti-HCV positive serum samples and anti-HCV negative but ALT abnormal serum samples were 94.0% and 100%, respectively.Conclusion The sensitivity and specificity of HCV-cAg ELISA is similar to that of HCV RT-PCR.The HCV-cAg ELISA has many advantages such as window-phase,decurtation, convenience and lower cost, which enable the assay a good clinically applied prospects for early diagnosis of hepatitis B.
