Influence of Tiaozhi Tongmai Granule on blood fat, RLP-C, sICAM-1 and sVCAM-1 in ApoE-/-mice
10.3969/j.issn.1006-2157.2018.03.006
- VernacularTitle:调脂通脉颗粒对ApoE基因敲除小鼠血脂、RLP-C、sICAM-1、sVCAM-1的影响
- Author:
Lu LI
1
;
Yangzhi GUO
;
Huimin YANG
;
Lichao QIAN
;
Cheng SHI
;
Shuyan WANG
;
Lei LIAO
Author Information
1. 北京中医药大学东直门医院 北京 100070
- Keywords:
blood fat;
triglyceride-rich remnant-like particle;
atherosclerosis;
ApoE-/-mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(3):210-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Tiaozhi Tongmai(Fat-regulating and Vessel-freeing) Granule (TZTMG) on blood fat, triglyceride-rich remnant-like particle (RLP) and cell adhesion molecule in ApoE-/-mice. Methods After established model of atherosclerosis(AS),ApoE-/-mice(n=30) were randomly divided into model group, fenofibrate group and TZTMG group (each n =10). C57BL/6J mice (n=10) were chosen into normal group. All medicinal groups were fed with high-fat diet, meanwhile were intervened with TZTMG and fenofibrate. The indexes of triglyceride-rich RLP (RLP-C) and 4 items of blood fat were detected detector after 12 weeks. The levels of serum intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were detected by using ELISA. The aortic root was collected to prepare paraffin sections for observing morphological changes af-ter HE staining. Results Since the 4thweek, mouse weight decreased in TZTMG group and fenofibrate group compared with model group. The levels of serum RLP-C, TC, TG, HDL-C and LDL-C increased in model group,fenofibrate group and TZTMG group compared with normal group(P<0.05). The lev-els of serum RLP-C and TG decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). The levels of ICAM-1 and VCAM-1 increased significantly in model group, fenofibrate group and TZTMG group compared with normal group (P <0.05). The levels of serum ICAM-1 and VCAM-1 decreased in fenofibrate group and TZTMG group compared with model group (P <0.05). Comparison in VCAM-1 among normal group,fenofibrate group and TZTMC group had no statistical sig-nificance. HE staining showed there were thickened aortic intima and vulnerable plaques in model group, and thickened aortic intima and stable plaques in TZTMG group. Conclusion TZTMG can decrease the expression of adhesion molecule and delay AS development through reducing RLP-C level.
