Investigation of rotavirus contamination in commercial oysters in Beijing
10.3760/cma.j.issn.1003-9279.2016.01.011
- VernacularTitle:北京地区市售牡蛎轮状病毒污染调查
- Author:
Zhiyong GAO
1
;
Hanqiu YAN
;
Baiwei LIU
;
Da HUO
;
Dandi LI
;
Haikun QIAN
;
Lijuan CHEN
;
Quanyi WANG
Author Information
1. 100013,北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室
- Keywords:
Oyster;
Group A rotavirus;
Semi-nested RT-PCR;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2016;(1):41-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the rotavirus contamination in commercial oysters in Beijing. Methods Between February 2014 and March 2015, a total of 280 oysters were collected in a large aquatic market in Beijing, and 5 oysters per stall were collected and classified as one sample.The samples were processed using three kinds of methods:direct treatment, PEG ( polyethylene glycol) 8 000 precipitation and proteinase K digestion-PEG 8 000 precipitation.Group A rotaviruses were detected by real time RT-PCR, and G/P genotyping was performed using the semi-nested RT-PCR.The VP7 and VP4 genes of positive samples were amplified, sequenced, and the phylogenetic tree was constructed using the maximum likelihood method with MEGA software ( version 6.06 ) .Results The detection rates of group A rotaviruses of three methods (direct treatment, PEG 8 000 precipitation and proteinase K digestion-PEG 8 000 precipitation) were 3.57%(2/56), 7.14%(4/56) and 5.38% (3/56), respectively.Rotaviruses were detected in 8 oysters samples ( 14.29%) , which were collected during autumn and winter . The G/P genotype combination included G9/P[8] (2 strains) and G9/P[N] (6 strains).The VP7 genes of 3 strains and the VP4 gene of one strain were sequenced successfully, and the phylogenetic analyses demonstrated that these strains had the highest similarity to those G9/P[8] strains prevailing in recent years in China.Conclusion Group A rotaviruses were detected in some commercial oysters during autumn and winter in Beijing, indicating a risk of foodborne illness.
