Study on the mucosal immune response induced by intranasal immunization with HPV16 and 18 L1 virus like particles plus JY adjuvant in cynomolgus
10.3760/cma.j.issn.1003-9279.2016.01.006
- VernacularTitle:HPV16与18型 L1病毒样颗粒联合 JY 佐剂鼻腔免疫食蟹猴诱导黏膜免疫应答
- Author:
Fenlian MA
1
;
Hai PAN
;
Zhihui CHENG
;
Huayue YE
;
Lishu ZHENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Human papillomavirus types 16 and 18 L1;
virus-like particle;
JY adjuvant;
Mucosal immunity
- From:
Chinese Journal of Experimental and Clinical Virology
2016;(1):23-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mucosal immunity of L1 virus-like particles ( VLPs) of human papillomavirus ( HPV) types 16 and 18 plus JY adjuvant by intranasal immunization in cynomolgus. Methods Cynomolgus were immunized with low and high dosage of HPV types 16 and 18 L1 VLP with JY adjuvant for 3 times by intranasal route at weeks 0, 4 and 8, respectively, using PBS as control. Subsequently, vaginal secretion, oral secretion and nasal secretion were collected at weeks 0, 2, 4, 6, 8 and 16, respectively, and determined for mucosal immunity by ELISA.Results HPV-L1-specific sIgA antibodies were detected in all secretions, including oral, nasal and vaginal ones, the concentrations of sIgA antibody induced were much higher than those in PBS control group.There was no significant difference ( P>0.05) in sIgA antibody levels among cynomolgus vaccinated with low and high dosage of L1 VLP, as was between oral and nasal secretion ( P >0.05 ) , However, the concentrations of sIgA antibody in vaginal secretion were significant higher than those in oral and nasal secretion, differences were statistically significant ( P<0.01) .Conclusions Following intranasal immunization in cynomolgus, HPV types 16 and 18 L1 VLP with JY adjuvant can effectively induce sIgA antibody in vaginal secretion, and vaginal sIgA antibody concentrations were much higher than those in oral and nasal secretion.
