Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells.
10.3858/emm.2009.41.9.069
- Author:
Ji Ae LEE
1
;
Ha Yong SONG
;
Sung Mi JU
;
Su Jin LEE
;
Hyung Joo KWON
;
Won Sik EUM
;
Sang Ho JANG
;
Soo Young CHOI
;
Jinseu PARK
Author Information
1. Department of Biomedical Science, College of Medicine, Hallym University, Chunchon 200-702, Korea. jinpark@hallym.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cyclooxygenase 2;
lipopolysaccharides;
NF-kappaB;
nitric oxide synthase type II;
reactive oxygen species;
superoxide dismutase
- MeSH:
Animals;
Cell Line;
Cyclooxygenase 2/*genetics/metabolism;
Cytokines/immunology;
*Gene Expression Regulation;
Lipopolysaccharides/immunology/metabolism;
Mice;
Mitogen-Activated Protein Kinase Kinases/metabolism;
NF-kappa B/metabolism;
Nitric Oxide/metabolism;
Nitric Oxide Synthase Type II/*genetics/metabolism;
Reactive Oxygen Species/metabolism;
Superoxide Dismutase/*metabolism
- From:Experimental & Molecular Medicine
2009;41(9):629-637
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat-SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE2 production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
