First isolation and identification of Banna virus from Culicoides pools in Yunnan
10.3760/cma.j.issn.02546450.2016.01.001
- VernacularTitle:云南库蠓中版纳病毒的首次分离及鉴定
- Author:
Meiling KOU
1
;
Jianbo ZHU
;
Heng YANG
;
Lei XIAO
;
Jinping WANG
;
Haisheng MIAO
;
Jinglin WANG
;
Huachun LI
Author Information
1. 云南省畜牧兽医科学院重点实验室
- Keywords:
Culicoides;
Banna virus;
Isolation;
Identification
- From:
Chinese Journal of Experimental and Clinical Virology
2016;(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the virus-carrying status and infection condition of Culicoides in Yunnan province.Methods Culicoides, cattle serum samples and pig serum samples were collected in Qujing City in Yunnan province from Sep.to Nov.in 2011; the supernatant of Culicoides was inoculated in C6/36 cells to isolate virus.Suspected isolates were identified by molecular biology techniques and titers of virus antibodies in pigs and cattles serum samples were tested by VN.Results 8 300 short tarsal Culicoides (8 300/18 160) and 7 100 Ryukyu Culicoides (7 100/18 160) were identified among 18 160 Culicoides specimens.Two suspected virus strains were isolated and designated as SC093 and SC233.The nucleotide sequence homology of VP2, VP4 and VP8 sequences with Banna virus Chinese strain ( Accession number:AF134526) is up to 99%.1 out of 200 (1/200) cattle serum samples was antibody positive against Banna virus with 0.5%positive rate.And the neutralizing antibody titers of SC093 and SC233 with above Banna virus antibody positive cattle serum were 160 and 40.10 out of 535 ( 10/535 ) pig serum samples were antibody positive against Banna virus with 1.7%positive rate.And the neutralizing antibody titers of SC093 and SC233 with above Banna virus antibody positive pig serum samples were 80-320 and 20-80 respectively.Conclusions The SC093 and SC233 virus strains isolated from Shizong were identified to be Banna virus and it could cause infection in pigs and cattles.This is the first report that Banna virus was isolated from Culicoides.
