Effects of baicalein on AC-cAMP-PKA signaling pathway in prefrontal cortex of rats with attentional deficit hyperactivity disorder
10.3969/j.issn.1006-2157.2018.02.006
- VernacularTitle:黄芩素对注意缺陷多动障碍大鼠前额叶皮质AC-cAMP-PKA信号通路的调控作用
- Author:
Qing WANG
1
;
Jinping FAN
;
Yingzhao LIAO
;
Jing CHEN
;
Hua LIU
;
Xinqiang NI
Author Information
1. 海南医学院中医学院 海南571199
- Keywords:
attentional deficit hyperactivity disorder;
baicalein;
AC;
cAMP;
PKA;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(2):124-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of baicalein on spontaneous activity, impulsivity, learning-memorial ability,and the adenylate cyclase(AC)-cyclic adenosine monophosphate(cAMP)-protein ki-nase A(PKA)signaling pathway in prefrontal cortex(PFC)of rats with attention deficit hyperactivity disorder(ADHD).Methods Fifty 4-week-old SHR rats were randomly divided into the model group,methylphenidate(MPH)group(2 mg/kg), and low-dose, mid-dose and high-dose(100, 200, 400 mg/kg)baicalein groups,and WKY rats of the same age were selected as the normal control group(n=10 in each group).Rats were orally gavaged for 4 weeks in the dark-phase.Open field test(OFT),ele-vated plus maze(EPM)and novel object recognition test(NORT)were conducted to evaluate the spon-taneous activity, impulsivity and learning-memorial ability of rats individually at the end of treatment. AC,cAMP and PKA quantity were determined with enzyme-linked immunosorbent(ELISA)method. Results Movement distance in OFT was reduced in high-dose baicalein group and mid-dose baicalein group(P<0.05)afterwards.Vertical movements also decreased in all baicalein groups.Percentage of the times entering the open arms in the EPM was significantly reduced in the mid-dose baicalein group(P<0.05);staying duration in the total period in the EPM was also reduced in all baicalein groups(P<0. 05).The preference index in the testing phase of NORT increased in the mid-dose baicalein group(P<0.05).Levels of AC and cAMP in the PFC increased in all baicalein groups(P<0.05), while tPKA increased in high-dose and mid-dose baicalein group(P<0.05).Conclusion Baicalein could increase the levels of AC,cAMP and PKA in PFC of ADHD rats, which may shed some light to the mechanism how baicalein could relieve the spontaneous activity and impulsivity,improve learning-memorial ability of ADHD rats.
