Evaluation of a rapid test kit for noroviruses of different genotypes
10.3760/cma.j.issn.1003-9279.2015.06.020
- VernacularTitle:一种快检试剂对不同基因型诺如病毒的检测评价
- Author:
Zhiyong GAO
1
;
Hanqiu YAN
;
Baiwei LIU
;
Da HUO
;
Yiwei DU
;
Quanyi WANG
Author Information
1. 100013,北京市疾病预防控制中心传染病地方病控制所食物中毒诊断溯源技术北京市重点实验室
- Keywords:
Norovirus;
Immunochromatography;
Rapid detection;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(6):547-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of a rapid immunochromatographic test kit for noroviruses of different genotypes in fecal specimens.Methods Between April 2011 and May 2015,101 norovirus-positive fecal specimens were collected from patients with diarrhea.Noroviruscs of genogroup Ⅰ (G Ⅰ) or Ⅱ (G Ⅱ) were detected using the real time RT-PCR.The capsid genes of noroviruses were amplified using the RT-PCR,and genotypes were identified through the phylogenetic analysis.The results of real-time RT-PCR and genotyping were employed as the reference,then the rapid immunochromatography test kit (RIDA(R) QUICK Norovirus) was evaluated.Results Among 101 specimens,8 strains of GⅠ and 93 strains of G Ⅱ were identified by real time RT-PCR,whereas 1 strains of GⅠ and 57 strains of G Ⅱ were identified by RIDA(R) QUICK Norovirus,with the coincidence rate of 57.4％.RIDA(R) QUICK Norovirus could detect GⅠ.9,G Ⅱ.3,G Ⅱ.4,G Ⅱ.6,G Ⅱ 7,G Ⅱ.13,G Ⅱ.17,G Ⅱ.21 and G Ⅱ.N,and it failed to detect GⅠ.2,GⅠ.6,GⅠ.N and G Ⅱ.2.The detection rates for G Ⅱ.4 strains and new G Ⅱ.17 variants were 77.8％ (28/36) and 38.1％ (8/21) respectively.Conclusion This assay was effective for common GⅡ noroviruses,but less reliable for new G Ⅱ.17 variants.
