Preparation of maternal strain X-157 HA antiserum for influenza virus
10.3760/cma.j.issn.1003-9279.2015.06.018
- VernacularTitle:流感病毒经典重配中X-157母本株HA抗血清制备
- Author:
Jing TANG
1
;
Li XIN
;
Junfeng GUO
;
Yuelong SHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Influenza Virus;
Classical Reassortment;
HA Protein;
High Immunogenicity;
HA Antiserum
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(6):540-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective Preparation of maternal strain X-157 HA antiserum for influenza virus classical reassortment.Methods X-157 virus was digested by bromelain after inactivation and purification.Sucrose continuous density gradient centrifugation method was used to purify HA protein.SDS-PAGE method was used to select the right protein band.Rabbit antiserum was collected after immunization.Antiserum HI titer was identified by HI method.Antiserum purity was identified by SIRD method.Results High Immunogenic X-157 HA protein was successfully prepared and the titer of anti-HA protein by HI reached to 2560.High purity HA antiserum was identified by SIRD method.Conclusions The key reagent in the classical reassortment of influenza virus was prepared,and the complete set of technical methods were explored,which laid the foundation for the independent research and development of seasonal influenza vaccine strains of China.
