Influence of medicated serum of Liuwei Dihuang Tang on PHD2/HIF-1α signal pathway in HK-2 cells
10.3969/j.issn.1006-2157.2018.01.010
- VernacularTitle:六味地黄汤含药血清对HK-2细胞PHD2/HIF-1α信号途径的影响
- Author:
Qun TANG
1
;
Hua WU
;
Chunyan LIU
;
Lin LUO
;
Chun YI
;
Wenfeng XU
;
Gaojian ZHUANG
Author Information
1. 湖南中医药大学病理教研室 湖南410208
- Keywords:
HK-2 cells;
renal interstitial fibrosis;
proline hydroxylase 2;
hypoxia-inducible factor 1α;
connective tissue growth factor;
medicated serum of Liuwei Dihuang Tang(Six-Ingredient Rehmannia Decoction);
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(1):60-64,75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PHD2/HIF-1 signaling pathway on the renal interstitial fibrosis,and the intervention effect of medicated serum of Liuwei Dihuang Tang on HK-2 cells induced by hypoxia.Methods A total of 40 SPF male SD rats were divided into blank group and medicated group(each n=20).The blank group was orally given normal saline 〔10 mL/(kg· d)〕 and medicated group was orally given Liuwei Dihuang Tang for 1 week continuously, and then blood samples were collected from rat abdominal aorta for separating serum after centrifugation.HK-2 cells were cultured in vitro.HK-2 cells were divided into blank control group(10% of fetal bovine serum), normal group (10%of normal serum),CoCl2group(150 μmol/L of CoCl2+10% of normal serum)and medicated serum group(150 μmol/L of CoCl2+10% of medicated serum of Liuwei Dihuang Tang).HK-2 cells were cultivated in vitro and treated in all groups for 24 h,and then the influence of medicated serum of Liuwei Dihuang Tang on protein levels and mRNA expressions of PHD 2, HIF-1αand connective tissue growth factor(CTGF)in HK-2 cells were detected,respectively,by using Western blotting method and reverse transcription polymerase chain reaction(RT-PCR).Results Compared with normal group, the protein level and mRNA expression of PHD 2 in HK-2 cells all decreased(P <0.05), while protein levels of HIF-1αand CTGF and mRNA expressions of HIF-1αand CTGF increased(P<0.05)in CoCl2 group.Compared with CoCl 2group, the protein level and mRNA expression of PHD2 increased(P<0.05),and those of HIF-1αand CTGF decreased(P<0.05)in medicated serum group.Conclusion PHD2/HIF-1αsignal pathway takes part in RIF, and Liuwei Dihuang Tang can inhibit RIF through up-regulating PHD2 expression,improving HIF-1αdegradation and down-regulating CTGF expression.
