Molecular epidemiological investigation of adenovirus in hospitalized children associated with acute lower respiratory infection in Changsha from 2013 to 2014
10.3760/cma.j.issn.1003-9279.2015.06.010
- VernacularTitle:2013-2014年长沙急性下呼吸道感染住院儿童腺病毒的分子流行特征
- Author:
Xiaofang LUO
1
;
Bing ZHANG
;
Zhaojun DUAN
;
Qin LIU
;
Zhiping XIE
Author Information
1. 湖南师范大学第一附属医院儿童医学中心
- Keywords:
Acute lower respiratory infection;
Children;
Adenovirus,human;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(6):505-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epidemiological features and the types of human adenovirus (HAdV) in hospitalized children with acute lower respiratory tract infection (ALRTI) in Changsha from 2013 to 2014.Methods Six hundreds and three nasopharyngeal aspirates were collected from the children with ALRTI,who were hospitalized at the pediatric of People' s Hospital of Hunan Province from April 2013 to March 2014.Eleven kinds of respiratory virus were firstly detected by Real-time PCR,the HAdV-positive samples are typed by Nested-PCR based on the sequence of hexon gene.The sequences were analyzed and compared with GenBank data.Results Adenovirus was detected in one hundreds and nineteen samples from six hundreds and three samples and the rate is 19.73％.Among the eighty eight samples that successful classified,twenty seven samples for HAdV-7 (30.68％),seventeen for HAdV-2 (19.32％),fourteen for HAdV-3 (15.91％),fourteen for HAdV-1 (15.91％),six for HAdV-5 (6.82％),3 for HAdV-6(3.41％),four for HAdV-4(4.55％),two for HAdV-57(2.27％),one for HAdV-14 (1.14％).HAdV infection could occur in any season,and the peak season was summer.The vulnerable groups for HAdV were younger than seven years old.Conclusions HAdV is one of the most important pathogens of acute lower respiratory infection in children in Changsha,the most prevalent types of adenovirus were HAdV-7 and HAdV-2.The pathogenicity of HAdV-3 and HAdV-7 in B species was stronger than other types in C and E species.
