Effect of ethanol extract from Machixian on colon cancer cells and their stem cell proliferation in vitro:a mechanism study
10.3969/j.issn.1006-2157.2018.01.007
- VernacularTitle:马齿苋醇提取物对结肠癌细胞及其干细胞体外增殖作用的机理研究
- Author:
Yihong XIONG
1
;
Chao DENG
;
Wen BAI
;
Yu LIU
;
Xiaoqin YAO
;
Heiying JIN
Author Information
1. 北京大学国际医院中医科 北京102206
- Keywords:
Machixian(Herba Portulacae;
Portulaca);
colon cancer cells;
colon cancer stem cells;
apoptosis;
Notch signal pathway;
Wnt signal pathway
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(1):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the influence of ethanol extract from Machixian(Herba Portulacae, Portulaca)on colon cancer cells and their stem cell proliferation in vitro and effective mechanism. Methods The stem cells of colon cancer were cultivated with serum free medium.The inhibitory effects of ethanol extract from Machixian in difference doses on growth of HT-29 cells and HT-29 stem cells were detected by using CCK-8 assay.The influence of ethanol extract from Machixian on apoptosis and cell cycle of HT-29 cells and HT-29 stem cells was detected by using flow cytometer.The influence of ethanol extract from Machixian on genetic and protein expressions of Notch 1, Notch2 and β-catenin in Notch signal pathway and Wnt signal pathway of HT-29 cells and HT-29 stem cells was analyzed by using reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting method.Results The ethanol extract from Machixian had inhibitory effect on proliferation of HT-29 cells and HT-29 stem cells,its effective time and dose were positively correlated to cellular proliferation inhibition rate, and its inhibitory effect on proliferation and improve effect on apoptosis rate were more significant on HT-29 cells than those on HT-29 stem cells(P<0.05).The ethanol extract from Machixian could down-regulate genetic expressions of Notch1 and β-catenin and protein expressions of Notch 1,Notch2 and β-catenin of HT-29 cells and HT-29 stem cells(P<0.05).Conclusion The ethanol extract from Machixian can inhibit in vitro the proliferation of colon cancer cells and their stem cells through down-regulating protein expressions of Notch1,Notch2 and β-catenin.
