Lentiviral vectors of MicroRNA-126 enhance the function of MSCs-hydrogel on mandibular bone defect
10.3760/cma.j.issn.1003-9279.2015.06.003
- VernacularTitle:MicroRNA-126慢病毒增强MSCs-水凝胶修复下颌骨缺损效果的研究
- Author:
Fucai SUN
1
;
Yike MA
;
Zhibin CAI
;
Zhaoan YANG
Author Information
1. 325000,温州医科大学附属第一医院口腔科
- Keywords:
MicroRNA-126;
lentiviral vector;
mesenchymal stem cell;
osteoblast
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(6):475-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of lentiviral vector of MicroRNA-126 on survival differentiation of mesenchymal stem cells under ischemic condition.Methods Lentiviral vector of MicroRNA-126 was constructed and then transfected into mouse mesenchymal stem cells (MSCs).Flow detection was used to detect transfection ability and synthesis of collagen type Ⅰ.ELISA was used to detect osteocalcin,Insulin-like growth factor 1 (IGF-1) and transforming growth factor-β (TGF-β) in cell culture supernatant.Mandibular bone defect model was constructed and then injected the hydrogel mixed with MSCs.After 48h,the survival rate and proportion of differentiation into osteoblasts was determined.Results Flow results showed lentiviral vector of MicroRNA-126 could transfected into MSCs.ELISA results showed that MicroRNA-126 lentiviral vector transfected MSCs secreted more bone gla protein and IGF-1 and TGF-β,which increased by 1.43,1.87 and 2.63 times compared with the control virus treated group.Animal experiments showed the number of stem cells survived in the MicroRNA-126 group was 4.56 times that of the control group and the percentage of MSC differentiation into osteoblasts was 75％,which is 3.95 times more than that in the control group.Conclusion The research shows that lentiviral vector of MicroRNA-126 could maintain MSCs survival and differentiation under ischemic condition.
