Mechanism of Yixin Jiedu Fang in inhibiting apoptosis of H9C2 cells
10.3969/j.issn.1006-2157.2018.01.005
- VernacularTitle:益心解毒方抑制H9C2心肌细胞凋亡的作用机制
- Author:
Hong CHANG
1
,
2
;
Yong WANG
;
Chun LI
;
Kuiyuan HUO
;
Wei WANG
Author Information
1. 北京中医药大学 北京100029
2. 华北理工大学
- Keywords:
H9C2 myocardial cells;
Yixin Jiedu Fang(Supplementing Heart and Removing Toticity De-coction);
apoptosis
- From:
Journal of Beijing University of Traditional Chinese Medicine
2018;41(1):25-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and possible mechanism of Yixin Jiedu Fang Supplementing Heart and kemoving Toticity Decoction in inhibiting apoptosis of H 9C2 myocardial cells.Methods H9C2 cells were divided into normal group,model group,Yixin Jiedu Fang group and quercetin group. The apoptosis of H9C2 myocardial cells was induced by using hypoxia-hypoglycemia/reoxygenation-reglycemia method,and protective effect of Yixin Jiedu Fang was studied.The survival rate of H9C2 cells was detected by using CCK-8 assay.The morphological changes of H9C2 cells were observed with microscope.Intracellular calcium overload was detected by using Fluo-3AM method and apoptosis of H9C2 cells was detected by using Hoechst 33342 method.The genetic expressions of Fas and FasL were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-PCR), and expressions of p-Akt protein and caspase-8 protein were detected by using Western blotting method. Results Compared with normal group, the survival rate of H9C2 cells decreased, cell shrinkage and deformation,intracellular calcium overload and more apoptosis cells were observed, expressions of Fas mRNA and FasL mRNA increased, expression of caspase-8 protein increased, and expression of p-Akt protein decreased in model group(all P<0.05).Compared with model group,the survival rate of H9C2 cells increased, cellular morphology were improved, intracellular calcium overload was relieved, expression of p-Akt protein was up-regulated,and expressions of FasL mRNA and caspase-8 protein were down-regulated in Yixin Jiedu Fang group(all P<0.05).Conclusion Yixin Jiedu Fang can inhibit the apoptosis of H9C2 cells, and the mechanism maybe related to the relief of intracellular calcium overload and control of exogenous apoptotic pathway.
