Association between D32E/M28V + L94P variant of Human papilloma virus type 16 E6/E7 gene with CIN and the cervical cancer
10.3760/cma.j.issn.1003-9279.2015.05.008
- VernacularTitle:HPV16 E6/E7基因变异体D32E/M28V+L94P与CIN和宫颈癌的相关性
- Author:
Jie REN
1
;
Qizhu ZHANG
;
Yingjie YANG
;
Dan ZI
;
Jianjiang ZHOU
;
Yujie TAN
Author Information
1. 贵州医科大学附属医院妇产科
- Keywords:
Papillomavirus,human virus;
Genotype;
Mutation (genetics);
Cervical disease
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(5):417-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to determine the distribution of HPV-16 E6/E7 genetic variation in patients with invasive cervical cancer or pre-cancer and normal person.Methods HPV infection was detected through flow-through hybridization and gene chip techniques to determine the prevalence of HPV 16 E6/E7 genetic variation.HPV16 E6/E7 gene was amplified by PCR,and the gene was purified by gel and then sequenced bi-directional.Results The rates of DNA sequence mutation and amino acid mutation were 76.5％ (62/81) and 66.7％ (54/81),respectively.Both E6 and E7 genes showed higher mutation rate than their prototypes.The prevalence of E6/E7 mutation significantly differed between the cervical cancer and the controls (P ＜ 0.05) and also between the cervical precancer and the controls (P ＜ 0.05).Mutations were simultaneously detected at the E6-D32E (T96A) and E7-M28V (A82G)/L94P (T281C) sites of the amino acid sequence.The most common genetic variation was D32E/M28V/L94P,which accounted for 35.8％ of the cases (29/81).D32E/M28V/L94P mutation was higher in the cervical cancer and pre-cancer compared with the controls (P ＜ 0.05).D32E/M28V/L94P mutation was higher in the cervical cancer and pre-cancer compared with the prototype,but there were no difference between the cervical cancer and the cervical pre-cancer(P ＞ 0.05).Conclusions HPV-16 E6/E7 genetic variations,such as D32E/M28V/L94P,was associated with cervical cancer or pre-cancer.HPV-16 E6/E7 genetic variations has no difference in the degree of cancer igenesis in the cervical cancer and pre-cancer.
