Association between TRAF6 gene polymorphisms and risk and inflamma-tory reactions of stroke with wind-phlegm stasis pattern
10.3969/j.issn.1006-2157.2017.12.011
- VernacularTitle:肿瘤坏死因子受体相关因子6基因多态性对中风风痰瘀阻证发生风险及炎性反应过程的影响
- Author:
Lian GU
1
;
Ziwen CHEN
;
Li SU
;
Yan YAN
;
Guangliang WU
;
Baoyun LIANG
;
Jinjing TAN
;
Nong TANG
Author Information
1. 广西中医药大学第一附属医院 广西530023
- Keywords:
stroke;
TRAF6;
single nucleotide polymorphism;
mRNA;
inflammatory cytokines
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(12):1030-1036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between tumor necrosis factor receptor-associated factor 6 ( TRAF6 ) gene polymorphisms and susceptibility to stroke with diverse TCM patterns and inflam-mation-related quantitative characters. Methods A case-control design was applied in this study. 311 stroke cases with the pattern of wind-phlegm stasis and 284 cases with qi-deficiency blood-stasis pattern were included in the case group, and 605 gender-matched, and age-matched cases were enrolled in the control group. The expression level of TRAF6 mRNA was determined by using real-time PCR ( RT-PCR), and two single nucleotide polymorphisms (SNP) of the TRAF6 gene, rs5030411 and rs5030416, were genotyped with Sequenom MassARRAY iPLEX platform. Enzyme-linked immuno sorbent assay ( ELISA) was applied to detect the serum levels of inflammatory cytokines. PLINK and SPSS software were used for statistical analysis. Results The TRAF6 mRNA expression level in patients with wind-phlegm stasis pattern or qi-deficiency blood-stasis pattern were significantly higher than those in the con-trol group ( P<0 . 050 ) . Rs5030416 polymorphism has a close association with susceptibility to stroke with wind-phlegm stasis pattern ( P =0 . 030 ) . No relationship was found between rs5030411 polymor-phism and stroke susceptibility ( P>0 . 050 ) . Patients carrying rs5030411-CT genotype in wind-phlegm stasis pattern group had remarkably higher interleukin-1b (IL-1b) and interleukin-6 (IL-6) levels than other genotype carriers. Moreover, compared with control group, the levels of IL-1b and IL-6 were signif-icantly higher in wind-phlegm stasis pattern group ( P<0 . 05 ) . Conclusion TRAF6 gene polymorphism rs5030416 may play a role in the occurrence of wind-phlegm stasis pattern, and rs5030411 polymorphism may engage in the inflammatory reaction in wind-phlegm stasis pattern.
