Study novel H7N9 avian influenza virus variationin in Wuxi area
10.3760/cma.j.issn.1003-9279.2015.05.003
- VernacularTitle:无锡地区新型H7N9禽流感病毒基因变异分析
- Author:
Xia LING
1
;
Guangyuan MA
;
Jing BAO
;
Yong XIAO
;
Yanhua QIAN
;
Shanhui CHEN
Author Information
1. 214023,无锡市疾病预防控制中心检验部
- Keywords:
Avian influenza;
H7N9 avian influenza;
Hemagglutinin;
Neuraminidase
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(5):396-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate genetic changes in hemagglutinin (HA) and neuraminidase (NA) protein sequences of novel H7N9 during the first and second outbreak in Wuxi.Methods RNA was extracted from patients and environment samples for detecting target gene.Gene sequencing of HA and NA was performed for the PCR amplicons.Phylogenetic and molecular characterizations were analyzed by Bioedit and MEGA software.Results Gene comparison showed that the similarity in the nucleic acids of HA found in the two outbreaks ranged from 99.09％ to 99.55％,amino acid similarity of 99.09％ to 99.55％,except for the substitution of Ell6G,Q154R,L213S,C421Y,M475I in the second outbreak.Meantime,the amino acid similarity of NA was 99.09％-99.77％.The phylogenetic tree indicated that environment strain appeared in both of the two clusters.Conclusions Phylogenetic and molecular characterizations suggest that the infection of novel H7N9 avian influenza virus has not been prevalent among public in Wuxi.
