Technique of detection of hepatitis c virus core antigen used in safety blood transfusion
10.3760/cma.j.issn.1003-9279.2015.04.027
- VernacularTitle:丙型肝炎病毒核心抗原检测技术在安全输血中的应用价值
- Author:
Yongping JI
1
;
Bin ZHOU
;
Haiyong MA
;
Aihua SUN
Author Information
1. 323000,丽水 浙江省丽水市中心血站
- Keywords:
Hepatitis viruses;
Blood donors;
Hepatitis C antigens
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(4):371-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility to screen donor with hepatitis C virus (HCV) infection by HCV core antigen ELISA (HCV-cAgELISA).Methods 183 anti-HCV positive,6370 anti-HCV negative (including 120 specimens with HbsAg +) serum specimens by using HCV-cAgELISA and HCV RT-PCR methods.Results Comparing with HCV RT-PCR,the detection results of HCV-cAgELISA consistency were 92.34％ in anti-HCV positive serum specimens and 99.97％ in anti-HCV negative serum specimens,respectively.In 6370 anti-HCV negative specimens,1 serum sample was positive HCV RNA identification in 3 serum specimens which were positive for HCV-cAgELISA.HCV RNA copies and HCV-cAg positive rate was a positive correlation.Conclusion Sensitivity of HCVcAg ELISA is silllilar to HCV RT-PCR.HCVcAgELISA is a simple,fast,and reliable memod to screen donor with HCV infection in blood transfusion medicine.
