Extraction of polysaccharides of Luotuopeng (Peganum harmala L.,Herb of Common Peganum) and its effect on toxicity of PolyQ accumulation in Caenorhabditis elegans
10.3969/j.issn.1006-2157.2017.11.014
- VernacularTitle:骆驼蓬多糖提取及对秀丽隐杆线虫多聚谷氨酰胺聚集毒性的影响
- Author:
Qinghua QUAN
1
;
Ruifang JI
;
Jiang YUAN
;
Jiali WANG
;
Yibing LIU
;
Yuan ZOU
;
Xiaoyu GUO
;
Jiamei ZHANG
;
Yonggang LIU
;
Zengping GAO
Author Information
1. 北京中医药大学中药学院 北京100102
- Keywords:
polysaccharides of Luotuopeng (Peganum harmala L.,Herb of Common Peganum);
content determination;
Caenorhabditis elegans;
Huntington's diseases;
PolyQ
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(11):954-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe into the influence of polysaccharides of Luotuopeng (Peganum harmala L.,Herb of Common Peganum) on toxicity of PolyQ accumulation in the muscle cells of AM141 strain of Caenorhabditis elegans (C.elegans).Methods The polysaccharides of Luotuopeng were extracted by using water extraction followed by sequential alcohol deposition method,and the content of each precipitation was determined by using phenol-sulfuric acid method.The polysaccharides were added to medium,and then samples were collected for counting the number of fluorescence dots due to PolyQ-YFP accumulation in the muscle cells of C.elegans.The influence of the polysaccharides on toxicity of PolyQ accumulation in AM141 strain of C.elegans was reviewed.The precipitation precipitated by 60％ ethanol with higher activity was purified by using macroporous resin for re-reviewing the activity.Results The mass fractions of the polysaccharides precipitated by 30％ ethanol,60％ ethanol or 80％ ethanol were,respectively,20.07％,21.80％,19.98％.The inhibition rates of the polysaccharides on the fluorescence dots due to PolyQ-YFP accumulation in the muscle cells of C.elegans were,respectively,7.9％,17％ and-1.72％.After the precipitation precipitated by 60％ ethanol purified with macroporous resin,the mass fractions of the polysaccharides,in 10％ and 20％ ethanol elution with higher mass fractions,were,respectively,49.76％ and 38.26％,and the inhibition rates were,respectively,35.25％ and 18.03％.Conclusion The method for reviewing the influence of the polysaccharides on toxicity of PolyQ accumulation through model of AM141 strain of C.elegans is easy,stable and reliable.The polysaccharide precipitated by 60％ ethanol has the highest inhibitory effect on PolyQ accumulation with a dose-effect relationship.
