Influence of Bugan Jianyao Fang (Liver-tonifying Lumbar-invigorating Decoction) on expressions of MMP-3 and TIMP-1 in rats with lumbar disc degeneration
10.3969/j.issn.1006-2157.2017.11.012
- VernacularTitle:补肝健腰方对腰椎间盘退变大鼠椎间盘MMP-3、TIMP-1表达的影响
- Author:
Xiangzhong QIU
1
;
Dong LIU
;
Xincheng ZHANG
;
Xuyi TAN
;
Hao TANG
;
Shengchang JIANG
Author Information
1. 湖南省中医药研究院附属医院 湖南410006
- Keywords:
Bugan Jianyao Fang (Liver-tonifying Lumbar-invigorating Decoction);
intervertebral disc;
degeneration;
matrix metalloproteinase-3;
tissue inhibitor of metalloproteinase-1;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(11):940-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of Bugan Jianyao Fang (Liver-tonifying Lumbarinvigorating Decoction) on expressions of intervertebral disc matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with lumbar disc degeneration.Methods SD rats (n =75) were randomly divided into sham-operation group,model group,Yaobitong (Waist Impediment-Freeing Capsules) group,doxycycline (DOX) group and Bugan Jianyao Fang group (each n =15).Mter establishing successfully the model of lumbar disc degeneration in rats,all groups were,respectively,given orally Yaobitong,DOX and Bugan Jianyao Fang.The expressions of intervertebral disc MMP-3 and TIMP-1 were detected in all group after and 20 d and 4 d after intervention.Results After intervention for 20 d and 40 d,the expressions of intervertebral disc MMP-3 and TIMP-1 decreased in Yaobitong group,DOX group and Bugan Jianyao Fang group compared with model group (P ＜0.05).The difference in expressions of intervertebral disc MMP-3 and TIMP-1 had statistical significance in Yaobitong group,DOX group and Bugan Jianyao Fang group after intervention compared with those in the same groups before intervention (P ＜ 0.05).The comparison among Bugan Jianyao Fang group,Yaobitong group and DOX group showed that the difference had no statistical significance (P ＞ 0.05) at all time points.Conclusion Bugan Jianyao Fang can reduce the expressions of intervertebral disc MMP-3 and TIMP-1 in rats with lumbar disc degeneration,which may be one of mechanisms of Bugan Jianyao Fang preventing and curing lumbar disc degeneration.
