Regulation on autophagy with tanshinone ⅡA for anti-oxidative stress damage of endothelial cells through PI3K/Akt/mTOR pathway
10.3969/j.issn.1006-2157.2017.11.011
- VernacularTitle:丹参酮ⅡA通过PI3K/Akt/mTOR信号通路调控自噬抗内皮细胞氧化应激损伤研究
- Author:
Huimin CAO
1
;
Nan SONG
;
Ni ZHANG
;
Guanlin YANG
;
Wenna CHEN
;
Zhe ZHANG
;
Lianqun JIA
Author Information
1. 辽宁中医药大学中医脏象理论及应用教育部重点实验室辽宁省中医转化医学研究中心 辽宁110847
- Keywords:
tanshinone ⅡA;
oxidized low-density lipoprotein;
atherosclerosis;
oxidative stress;
autophagy
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(11):933-939
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective and mechanism of tanshinone Ⅱ A (TS Ⅱ A) in oxidative stress damage of endothelial cells induced by oxidized low-density lipoprotein (ox-LDL) based on PI3K/Akt/mTOR autophagy pathway.Methods EA.hy926 cells were cultured in vitro,and then randomly divided into normal group,model group,ox-LDL + TS Ⅱ A group,ox-LDL + TS Ⅱ A + LY294002 group,TS Ⅱ A group and LY294002 group.The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected by using chromatoptometry,autophagy status was observed by using FITC-LC3 fluorescence microscope and Naolive 3D Cell Explorer Real-time Microscopic Imaging System,and meanwhile,expressions of autophagy-related proteins and PI3K/Akt/mTOR pathway proteins were detected by using Western Blotting.Results Compared with normal group,MDA content increased,SOD activity decreased and content of LC3-Ⅰ/LC3-Ⅱ protein increased in model group (P ＜0.01),and after TS Ⅱ A intervention,MDA content decreased,SOD activity increased and content of LC3-Ⅰ/LC3-Ⅱ protein increased (P ＜ 0.01).Compared with normal group,protein expressions of PI3K,p-Akt and p-mTOR decreased significantly in model group (P ＜ 0.05 or P ＜ 0.01).Compared with model group,protein expressions of PI3K,p-Akt and p-mTOR decreased significantly after TS ⅡA intervention (P ＜0.05 or P ＜0.01).Compared with ox-LDL + TS Ⅱ A group,content of LC3-Ⅰ/LC3-Ⅱ protein decreased and autophagy level decreased (P ＜ 0.01) after applying LY294002,an inhibitor of PI3K.Conclusion Tanshinone ⅡA may improve autophagy through regulating PI3K/Akt/mTOR pathway to relieve the oxidative stress damage of EA.Hy926 cells and prevent atherosclerosis.
