Study on antitumor effect of DC vaccine containing mutant of HPV16 E6 E7 fusion gene

VernacularTitle:含HPV16 E6E7突变融合基因DC疫苗的抗肿瘤效果研究
Author: Dan LI ¹ ; Shanshan GUAN ; Qiang XIE ; Zhixiang ZHOU ; Zelin LI ; Yi ZENG
Author Information

1. 100124,北京工业大学生命科学与生物工程学院
Keywords: Papillomavirus,human; Mutation; Genes; Dendritic cells; Vaccines; Antigens,viral,tunor
From: Chinese Journal of Experimental and Clinical Virology 2015;29(4):338-341
CountryChina
Language:Chinese
Abstract: Objective To study the anti-tumor immune effect of the dendritic cells (DC) containing a codon optimized HPV16 E6E7 mutation fusion gene(HPV16 omfE6E7).Methods HPV16 omfE6E7 was generated by site-directed mutagenesis at L57G,C113R for the E6 protein and C24G,E26G,C58G for the E7 protein ofHPV16 of E6E7 fusion gene (HPV16 of E6E7),and a DC vaccine (DC-HPV16 omfE6E7E6E7) was constructed by infection of recombinant adenovirus expressing HPV16 omfE6E7E6E7 gene to prime dendritic cells.Results The HPV16 omfE6E7 gene constructed in this work showed a lost in the transformation capability to NIH 3T3 cells.DC vaccine containing HPV16 omfE6E7E6E7 antigen can stimulate specific CTL response,a significant decrease in the rate of tumor formation,and an intensively increase in the survival rate in the immunized mice.Conclusion The result indicated that the DC-HPV16 omfE6E7 vaccine constructed in this study has potential application to immunotherapy of HPV positive cancer.