Effects of electro-acupuncture on hippocampal CXCR4 and IP-10 protein expression in rats with depression disorder
10.3969/j.issn.1006-2157.2017.10.014
- VernacularTitle:电针对慢性应激抑郁模型大鼠海马CXCR4和IP-10蛋白表达的影响
- Author:
Xingchen LIANG
1
;
Huili JIANG
;
Danmei ZHANG
;
Rongxing SHI
;
Yu WANG
;
Jing LI
;
Xinjing YANG
;
Bingcong ZHAO
;
Qiuyun YU
;
Xiang LI
;
Shixing FENG
;
Ya TU
Author Information
1. 北京中医药大学针灸推拿学院 北京100029
- Keywords:
electroacupuncture;
depression disorder;
chronic stress;
CXCR4;
IP-10;
antibody microarrays technology;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(10):872-875
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electro-acupuncture (EA) on hippocampal chemokine cys-x-cys receptor 4 (CXCR4) and interferon-inducible protein-10 (IP-10) in rats with depression disorder.Methods The rat model of depression disorder was induced by unpredictable chronic-mild stimulation.Forty male rats were randomly divided into normal,model,EA and fluoxetine groups.Rats in EA group received electro-acupuncture at GV20 (Baihui) and GV29 (Yintang).Rats in fluoxetine group were administered with fluoxetine by oral gavage.On the 28th day,the expression of hippocampal CXCR4 and IP-10 were detected by using antibody microarrays technology.Fold change and ratio of the density of the proteins were used to investigate alteration of the differential protein expression.Results Compared with the normal group,unpredictable chronic-mild stimulation up-regulated the expression of hippocampal CXCR4 and IP-10 in model group (fold change =1.21,1.50).Compared with the model group,EA and fluoxetine reversed the up-regulation of hippocampal CXCR4 and IP-10 (EA group:fold change =0.65,0.72;fluoxetine group:fold change =0.57,0.62).Conclusion Effects of electro-acupuncture on depression disorder may be achieved by regulating the expression of hippocampal CXCR4 and IP-10.
