Effects of Danzhi Yin on mice with sequelae of pelvic inflammatory disease via JNK signal transduction pathway and inflammation-related cytokines

VernacularTitle:丹枝饮对盆腔炎性疾病后遗症小鼠JNK信号传导通路及相关炎性因子的影响
Author: Lin XU ¹ ; Yulong ZHUANG ; Zhe JIN
Author Information

1. 北京中医药大学北京100029
Keywords: Danzhi Yin; sequelae of pelvic inflammatory; JNK signal transduction pathway; MCP-1; MIP-2; mice
From: Journal of Beijing University of Traditional Chinese Medicine 2017;40(10):839-844
CountryChina
Language:Chinese
Abstract: Objective To investigate the mechanism of Danzhi Yin (Pony Bark Jasmine Fruit Decoction,DZY) in treating mice with sequelae of pelvic inflammatory disease (SPID).Methods 24 mice were randomly divided into the blank group,model group,DZY group and aspirin group.The SPID model was established by intrauterine infection with chlamydia muridarum.After treatment,serum levels of IL-6 and IL-8 were detected by using ELISA.The protein expression of MCP-1,MIP-2,and p-JNK were detected with Western blot.The protein phosphorylation of JNK was measured with immunochemistry technology.Results Compared with blank group,IL-6,IL-8,p-JNK,MCP-1,and MIP-2 levels in the model group were significantly higher (P ＜ 0.01).Compared with model group,IL-6,IL-8,p-JNK,MCP-1,and MIP-2 levels in the DZY group were significantly lower (P ＜ 0.01).Conclusion DZY may play an anti-inflammatory role by regulating the levels of cytokines (IL-6,IL-8) in serum.DZY may inhibit the inflammation through suppression of JNK signal transduction pathway.