Effects of Bushen Shengxue Jiedu Fang on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia
10.3969/j.issn.1006-2157.2017.10.008
- VernacularTitle:补肾生血解毒方对免疫介导的再生障碍性贫血小鼠淋巴细胞分化及血液学参数的影响
- Author:
Xue LI
1
;
Yi WEI
;
Peiying DENG
;
Bin DONG
;
Qiushuo JIN
;
Limin CHAI
Author Information
1. 北京中医药大学东直门医院中医内科学教育部和北京市重点实验室 北京100700
- Keywords:
aplastic anemia;
Bushen Shengxue Jiedu Fang;
hematological parameters;
lymphocyte differentiation;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(10):833-838
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Bushen Shengxue Jiedu Fang (Kidney-tonifying blood-generating toxin-removing Formula,BSSXJDF) on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia (AA).Methods Mice in all groups except the normal group received 60Co-γirradiation combined with injection of allogeneic immune cells in the tail vein to establish bone marrow hematopoietic inhibition model.The immunosuppressive cyclosporine A was used as the positive control drug,whereas Bushen Shengxue Jiedu Fang (BSSXJDF) was given to mice in the treatment groups for 28 consecutive days.Changes of hematological parameters after medication in the peripheral blood of mice were measured with cytometer at day 7,14,21 and 28.The ratio of T,B and NK cells was detected with flow cytometer.Results The count of red blood cells (RBC),hemoglobin (HGB),platelets (PLT) and white blood cells (WBC) in peripheral blood of mice were significantly increased (P ＜ 0.05) in BSSXJDF group compared with the model group;the ratio of CD3+ CD4+ cells in creased(P ＜ 0.01),and the ratio of CD3-CD19+ cells and CD3-NK1.1+ cells decreased (P ＜ 0.01) with statistical significance in the cyclosporine and BSSXJDF groups compored with the model group.Conclusion Bushen Shengxue Jiedu Fang can repair the hematopoietic function of bone marrow by regulating the differentiation of lymphocytes in the treatment of AA.
