Influence of heat-clearing dampness-resolving stasis-dispelling therapy on JAK/STAT signaling pathway mediated by Leptin in patients with chronic renal failure-damp-heat pattern
10.3969/j.issn.1006-2157.2017.09.012
- VernacularTitle:清热化湿祛瘀法对慢性肾衰竭湿热证患者瘦素介导的JAK/STAT信号通路的影响
- Author:
Dong WANG
1
;
Yiping WANG
;
Min YU
;
Shunjin HU
;
Yong LYU
;
Wan CHENG
;
Jiasheng LIU
;
Ling WEI
;
Hua JIN
Author Information
1. 安徽中医药大学第一附属医院 安徽230031
- Keywords:
chronic renal failure;
damp-heat pattern;
heat-clearing dampness-resolving stasis-dispelling therapy;
Qingshen (Kidney-Clearing) Granules;
serum Leptin;
Janus-activated kinase 2;
signal transducers and activator of transcriptions 3
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(9):777-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of Qingshen (Kidney-Clearing) Granules,with effects of clearing heat,resolving dampness and dispelling stasis,on expressions of Janus-activated kinase 2 (JAK2) and signal transducers and activator of transcriptions 3 (STAT3) in Janus-activated kinasesignaltransducer and activator of transcription (JAK/STAT) signaling pathway mediated by Leptin in patients with chronic renal failure (CRF)-damp-heat pattern,and investigate the mechanism of Qingshen Granules in treatment of CRF.Methods The patients with CRF-damp-heat pattern (n =68) were randomly divided into treatment group and control group (each n =34).The study was completed actually in 60 patients,and 29 in treatment group and 31 in control group.All 2 groups were given basic treatment of Western medicine,and treatment group was additionally given Qingshen Granules (thrice daily and 1 bas every time) for 12 weeks.The clinical efficacy and changes of serum creatinine (SCr),estimated glomerular filtration rate (eGFR),serum Leptin,JAK2 and STAT3 were observed in 2 groups.Results The clinical efficacy was 86.21％ (25/29) and total effective rate of TCM patterns was 82.76％ (24/29) in treatment group,and 58.06％ (18/31) and 54.84％ (17/31) in control group (P ＜0.05).After treatment,the level of SCr decreased significantly (P ＜ 0.01) and level of GFR increased significantly (P ＜ 0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P ＜ 0.05).After treatment,the level of Leptin,and expressions of JAK2 and STAT3 decreased significantly (P ＜ 0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P ＜ 0.05 or P ＜0.01).Conclusion Qingshen Granules can reduce the level of Leptin and expressions of JAK2 and STAT3,inhibit the activation of JAK/STAT signaling pathway mediated by Leptin,relieve clinical symptoms and improve kidney function in patients with CRF-damp-heat pattern.
