Technique optimization for extracting the total alkaloids from Baiqucai (Herba Chelidonii, Greater Celandine) by response surface analysis
10.3969/j.issn.1006-2157.2017.09.011
- VernacularTitle:响应面法优化白屈菜总生物碱提取工艺
- Author:
Hua JIANG
1
;
Jun LI
;
Xin LI
;
Haipeng DONG
;
Keyuan SHI
;
Dandan LIU
Author Information
1. 河南科技大学化工与制药学院 河南471023
- Keywords:
Baiqucai (Herba Chelidonii,Greater Celandine);
total alkaloids;
Plackett-Burman design;
Box-Behnken design;
extracting technique
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(9):772-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the best technique for extracting the total alkaloids from Baiqucai (Herba Chelidonii,Greater Celandine).Methods The yield of the total alkaloids was taken as review index by using acid dye colorimetry,and Plackett-Burman design (PBD) test was applied at first for screening 3 main factors influencing the technique of extracting the total alkaloids from Baiqucai and they were ethanol volume fraction,solid-liquid ratio and extracting times.Then the best technologic conditions for extracting the total alkaloids from Baiqucai were determined by applying response surface analysis (RSA) of Box-Behnken design (BBD) test.Results The best extracting technique was as follows:ethanol volume fraction was 85％,extracting duration was 60 min,extracting temperature was 70℃,degree of grinding was 40 meshes,solid-liquid ratio was 1:15,and extracting times were 3 times.In these conditions,the yield of the total alkaloids from Baiqucai was up to 1.92％,which was basically the same as theoretical prediction (1.95％).Conclusion The extracting technique optimized in this test is reasonable and feasible,and can get higher content of the total alkaloids.
