Volatile constituents of Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil) analyzed by applying solid phase microextraction-gas chromatography-mass spectrometry
10.3969/j.issn.1006-2157.2017.09.010
- VernacularTitle:零陵香挥发性成分的SPME-GC/MS分析
- Author:
Xueyang TANG
1
;
You QIN
;
Lin CHEN
;
Rongrong ZHOU
;
Xiangning SHAO
;
Shuyun SHI
;
Chunyu TANG
;
Shuihan ZHANG
Author Information
1. 湖南省中医药研究院中药研究所 湖南410013
- Keywords:
Linglingxiang (Lysimachia foenum-graecum Hance,Holy Basil);
volatile constituents;
solid phase microextraction;
gas chromatography-mass spectrometry
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(9):764-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the volatile constituents of Linglingxiang (Lysimachia foenumgraecum,Hance,Holy Basil) by applying solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC/MS).Methods The heads of solid phase microextraction (SPME) with 4 kinds of different coating,75 μm CAR/PDMS,65 μm DVB/PDMS,85 μm PA and 100 μm PDMS,were used to extract the under the best extraction condition,and then these volatile constituents were detected by using gas chromatography-mass spectrometry (GC-MS).Results The key parameters of SPME for extracting volatile constituents from Linglingxiang were as follows:temperature was 85℃C and extracting time was for 50 min.A total of 10 types including 103 kinds of volatile constituents were identified by 4 different SPME extraction heads,and phenols,esters and hydrocarbons were main chemical types of volatile compounds in Linglingxiang.There were totally 6 volatile constituents detected by 4 different SPME extraction heads,and they were Sugarlactone,Dihydroactinidiolide,(+)-cedrol,phytone,phenanthrene,and 3-Amino-4,5-dimethyl-2 (5h)-furanone.Among all extraction heads,the head of 75 μm CAR/PDMS detected the largest number of volatile constituents reached to 43.Conclusion SPME-GC/MS was used for the first time form determining the volatile constituents in Linglingxiang.The method is simple,rapid and accurate,which can offer reference to further studies on the material base of Linglingxiang.
