Influences of sonophoresis of Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment on synovial morphology and expressions of chondrocyte caspase-9 and XIAP in rabbits with knee osteoarthritis
10.3969/j.issn.1006-2157.2017.09.008
- VernacularTitle:超声导入复方三七消痛软膏对兔膝骨性关节炎滑膜形态和软骨细胞caspase-9、XIAP表达的影响
- Author:
Siqin FAN
1
;
Shan LI
;
Jie LIANG
;
Ruhui LIN
;
Qinglu LUO
Author Information
1. 福建中医药大学康复医学院 福建350122
- Keywords:
sonophoresis;
Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment;
knee osteoarthritis;
caspase-9;
X-linked inhibitor of apoptosis protein;
rabbits
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(9):750-757
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of sonophoresis of Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment on changes of synovial morphology and expressions of chondrocyte caspase-9 and X-linked inhibitor of apoptosis protein (XIAP) in rabbits with knee osteoarthritis (KOA).Methods New Zealand white rabbits (n =32,6 months old) were randomly divided into group of sonophoresis of Fufang Sanqi Xiaotong (sonophoresis group),group of coupling agent ultrasound treatment (coupling agent group),model group and normal group (each n =8).Except of normal group,other groups received anterior cruciate ligament transaction (ACLT).After 6 weeks,sonophoresis group and coupling agent group were given corresponding therapies for 2 treatment courses and model group and normal group were not given any interventions.The changes of synovial histomorphology were observed with light microscope after HE staining,and expressions of caspase-9 and XIAP were detected by using immunohistochemistry technique in all groups after 2 treatment courses.Results The degeneration degree of synovial membrane under light microscope showed an ascending order in all groups as follows:normal group,sonophoresis group,coupling agent group and model group.The value of integral optical density (IOD) of caspase-9 was higher,and IOD value of XIAP was lower in sonophoresis group,coupling agent group and model group than those in normal group.The IOD value of caspase-9 was lower,and IOD value of XIAP was higher in sonophoresis group and coupling agent group than those in model group.The IOD value of caspase-9 was lower,and IOD value of XIAP was higher in sonophoresis group than those in coupling agent group.Conclusion The sonophoresis of Fufang Sanqi Xiaotong is superior to ultrasound treatment,and has the protective effect on cartilage and relief effect on synovial inflammation,and can delay KOA development.The mechanism may be related to decrease of caspase-9 expression and increase of XIAP expression.
