Establishment of a mouse-adapted seasonal influenza A H3N2 virus strain and preliminary inquiry its molecular mechanism

VernacularTitle:流感病毒H3N2小鼠适应株的建立及分子机制探究
Author: Yunguang HU ¹ ; Juan XU ; Yajie HU ; Jie SONG ; Sudong QI ; Xiaofeng QIN ; Longding LIU
Author Information

1. 中国医学科学院北京协和医学院医学生物学研究所
Keywords: Influenza A virus; Mice,inbread BALBC; Vircs cultivastion
From: Chinese Journal of Experimental and Clinical Virology 2015;29(3):203-206
CountryChina
Language:Chinese
Abstract: Objective To establish adapted strain of seasonal influenza virus influenza A virus [A/ Hong Kong/1968 (H3N2)] in mice and explore the molecular mechanism of adaption preliminary.Methods The mice model was induced by nose dropping with wild type H3N2 influenza virus,by continuous passage in the lung of mice and detection of the clinical manifestation,viral load,virus titer,pathology,cytokines,to get the adapted strain of seasonal influenza A virus [A/Hong Kong/1968 (H3N2)].Results After 7 times continuous passage in the lung of mice,virulence was increased,viral load and virus titer were increased either.Genome sequencing and alignment indicated that the HA and NA gene was mutated.Conclusion The influenza virus H3N2 of mice lung adaption can be acquired from wild type seasonal H3N2 by continuous passage in the lung tissues of mice.Mutation of Ile to Val at residue 248 of HA and Val to Ile at residue 444of NA protein may be the key virulence determinant.