Effects of branched M2e polypeptides influenza A vaccine immunized through different routes
10.3760/cma.j.issn.1003-9279.2015.03.003
- VernacularTitle:甲型流感病毒分支状M2e多肽疫苗不同途径免疫效果的研究
- Author:
Lihong YAO
1
;
Xiaoyu LIU
;
Qi YANG
;
Aijun CHEN
;
Jianqiang GUO
;
Zhiqing ZHANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Peptides;
Vaccines,DNA;
Adjuvants,immanongic;
Immunity,mucosal;
Immunization,secondary;
Influenza A virus
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(3):199-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the immune effects induced with universal influenza A vaccine based on branched M2e polypeptides by two immunization routes in mice.Methods The M2e monomer and (M2e)4-tuftsin were synthesized using chemical method.One group of mice were immunized intramuscularly (i.m.) with 10 μg of (M2e)4-tuftsin plus aluminum adjuvant.And the other group of mice were intranasally immunized with the same amount of polypeptides plus JY adjuvant.Then the immune effects of different immune pathways were observed.Results Intramuscular route induced higher level of M2 specific antibody than intranasal route.Intramuscular injection of (M2e)4-tuftsin was the most effective in stimulating T cell responses.Both intramuscular and intranasal vaccinations (M2e) 4-tuftsin had protective effect,but intramuscular vaccination could provide the most efficient protective immunity.Conclusion The data indicated that the intramuscular vaccination could be the better way for immunization than intranasal vaccination.
