Protective effects of hedysarum polybotrys polysacchcaide on renal inter-stitid fibrosis in db /db mice with early diabetic nephropathy
10.3969/j.issn.1006-2157.2017.08.010
- VernacularTitle:红芪多糖对早期糖尿病肾病db/db小鼠肾间质纤维化的影响
- Author:
Zhisheng JIN
1
;
Yujiao WEI
;
Hailong LING
;
Huazhi ZHANG
Author Information
1. 甘肃中医药大学 甘肃 730000
- Keywords:
diabetic nephropathy;
HPS;
TGF-β1;
CTGF;
db /db mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(8):677-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of hedysarum polybotrys polysacchcaide (HPS)on the expression of transforming growth factor-β1 (TGF-β1 )and connective tissue growth factor (CTGF)as well as its kidney-protective mechanism in early diabetic nephropathy (DN)db /db mice.Methods 60 male mice at SPF 6 were used for this study.The above 50 db /db mice were divided into 5 groups (n =10 in each group)by using the random number table method:high-dose,mid-dose and low-dose HPS group (200,100,50 mg/kg,ig),telmisartan group (5 mg/kg,ig),and model group (distilled water of the same volume,ig).The remaining 10 db /m mice in the normal control group received equal vol-ume of distilled water via intragastric administration for 8 weeks.For mice in all groups,blood glucose levels were measured before the experiment and once every two weeks after intervention.24h urine was collected at the end of week 4 and 8 to measure urinary albumin levels by using ELISA method.Blood samples were drawn from the eyeball veins to measure the levels of serum creatinine (Scr)and blood urea nitrogen (BUN).Rat kidney tissue was dissected after sacrifice of these mice.Left kidney underwent HE staining to observe pathological changes in the renal cortex while the right kidney was used to detect the expression of TGF-β1 and CTGF mRNA and protein by using RT-PCR and Western blotting.Results Compared with the normal control group,blood glucose,Scr,BUN and 24 h urinary albumin levels in-creased significantly (P <0.01)in the model group;glomerular hypertrophy,mesangial widening,and capillary basement membrane thickening were observed.Expressions of TGF-β1 and CTGF mRNA and protein were significantly increased (P <0.01).Compared with the model group,blood glucose,Scr, BUN and 24 h urinary albumin levels decreased significantly (P <0.05)in all treatment groups except the low-dose HPS group (P >0.05);glomerular hypertrophy,mesangial widening and capillary base-ment membrane thickening presented with different degrees of improvement;expressions of TGF-β1 and CTGF mRNA and protein significantly decreased (P <0.01);curative effect of the mid-dose HPS group was superior to that of the telmisartan group.Conclusion HPS could prevent early diabetic nephropathy (DN)in db /db mice;its mechanism may be related to the inhibition of TGF-β1 and CTGF mRNA ex-pression level in the kidney.
